Detection of Point Mutations in Kirsten ras 2 Gene Using Locked Nucleic Acids Clamped PCR
Abstract
The aim of the study was to examine diagnostic possibilities of LNA (locked nucleic acids) -clamped PCR for detection of somatic mutations in the K-ras gene in 133 samples of human malignant colorectal tumors. Selective real-time PCR amplification of mutant alleles of the K-ras gene (codon 12 and 13) revealed the presence of mutations in 45 samples of the tumors. The detection limit of the used method for K-ras mutant alleles was 0.03 ng. LNA-clamped PCR suppressing amplification of the wild-type alleles of the K-ras gene provides a very sensitive and specific detection of mutations present in DNA samples of colorectal carcinomas.Downloads
Published
2007-10-15
How to Cite
Beránek, M., Bureš, J., Šácha, M., Sákra, L., Rajman, M., Jandík, P., Rudolf, E., & Landt, O. (2007). Detection of Point Mutations in Kirsten ras 2 Gene Using Locked Nucleic Acids Clamped PCR. Chemické Listy, 101(9). Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1748
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